Methotrexate-Coated Complexes of Plasmid DNA and Polyethylenimine for Gene Delivery
نویسندگان
چکیده
منابع مشابه
Ocular gene delivery systems using ternary complexes of plasmid DNA, polyethylenimine, and anionic polymers.
In this experiment, we developed anionic ternary complexes for effective and safe ocular gene delivery. Ternary complexes were constructed by coating plasmid DNA (pDNA)/polyethylenimine (PEI) complex with anionic polymers such as γ-polyglutamic acid (γ-PGA) and chondroitin sulfate (CS). The cationic pDNA/PEI complex showed high gene expression on the human retinal pigment epithelial cell line, ...
متن کاملEnhanced cellular uptake and gene delivery of glioblastoma with deferoxamine-coated nanoparticle/plasmid DNA/branched polyethylenimine composites.
Ternary composite nanomaterials based on deferoxamine-coated superparamagnetic iron oxide nanoparticles (8-10 nm), circular plasmid DNA (~4 kb) with fluorescent/luminescent reporter group, and branched polyethylenimine (25 kDa, PDI = 2.5) were prepared and compared in terms of their efficiencies in transfecting brain tumor cells at low concentration.
متن کاملPectin–Coated Polyethylenimine Polyplexes as Effective Gene Delivery Systems
Gene therapy is believed to be the therapy of the future because it aims to eradicate causes rather than symptoms of diseases. The generic aim of gene therapy is to deliver DNA inside cells in order to achieve a stable and regulated expression of transgenes in the target cells or tissues without side effects. Spontaneous entry of naked DNA inside cells is an inefficient process, consequently, a...
متن کاملSecure and effective gene delivery system of plasmid DNA coated by polynucleotide.
Polynucleotides are anionic macromolecules which are expected to transfer into the targeted cells through specific uptake mechanisms. So, we developed polynucleotides coating complexes of plasmid DNA (pDNA) and polyethylenimine (PEI) for a secure and efficient gene delivery system and evaluated their usefulness. Polyadenylic acid (polyA), polyuridylic acid (polyU), polycytidylic acid (polyC), a...
متن کاملPolyethylenimine-coated albumin nanoparticles for BMP-2 delivery.
Nanoparticle (NP)-based delivery has gained importance for improving the potency of therapeutic agents. The bovine serum albumin (BSA) NPs, obtained by a coacervation process, was modified by electrostatic adsorption of cationic polyethylenimine (PEI) to NP surfaces for delivery of bone-inducing growth factor, bone morphogenetic protein-2 (BMP-2). Different concentrations of PEI were utilized f...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 2018
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.b18-00144